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Health Organisation, Malaria Comission; World Health Organization : Organisation Mondiale de la Sante; Serie de Monographies, Issue No. 71. 749: A Preliminary Report on the Preparation and Trial of Multi-Species Antigen on Human Urias for Use in the Indirect Fluorescent Antibody Test

By Alexander J. Sulzer


Description
Medical Reference Publication

Excerpt
For serological diagnosis of human malaria by the indirect fluorescent antibody test, homologous antigens of Plasmodium must be used to detect the greatest percentage of malaria cases (Sulzer et al. 1969). Testing each serum with antigens of the four human malaria specles 1s time consuming and requires relatively large amounts of reagents. In several laboratories, a single simian malaria species has been used for determining human malaria antibody for both routine diagnosis and epidemiological studies. Use of only one species decreases the workload and the amount of reagents used, but it also decreases sensitivity of case detection. One multi-species antigen, composed of all the homologous human malaria s l c i o s , wr)illd decrease the test performance time as well as maintain the efficiency of antit) . Preparation of a rulti-species antigen of P, falciparum, P, vivax, and P, brasillanum and initial test results with this antigen are described below.

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